A deep insight into the sialome of male and female aedes aegypti mosquitoes

Only adult female mosquitoes feed on blood, while both genders take sugar meals. Accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. We performed de novo assembly of reads from adult Aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). By mapping back the reads to the assembled contigs, plus mapping the reads from a publicly available Ae. aegypti library from adult whole bodies, we identified 360 transcripts (including splice variants and alleles) overexpressed tenfold or more in the glands when compared to whole bodies. Moreover, among these, 207 were overexpressed fivefold or more in female vs. male salivary glands, 85 were near equally expressed and 68 were overexpressed in male glands. We call in particular the attention to C-type lectins, angiopoietins, female-specific Antigen 5, the 9.7 kDa, 12–14 kDa, 23.5 kDa, 62/34 kDa, 4.2 kDa, proline-rich peptide, SG8, 8.7 kDa family and SGS fragments: these polypeptides are all of unknown function, but due to their overexpression in female salivary glands and putative secretory nature they are expected to affect host physiology. We have also found many transposons (some of which novel) and several endogenous viral transcripts (probably acquired by horizontal transfer) which are overexpressed in the salivary glands and may play some role in tissue-specific gene regulation or represent a mechanism of virus interference. This work contributes to a near definitive catalog of male and female salivary gland transcripts from Ae. aegypti, which will help to direct further studies aiming at the functional characterization of the many transcripts with unknown function and the understanding of their role in vector-host interaction and pathogen transmission

Depolarization channel for barcelona lidar. Implementation and preliminary measurements

A new depolarization channel has beenimplemented in the BarcelonaTech University(UPC) multi-wavelength lidar system. The opticaland mechanical designs are presented. The specialconfiguration of the total power channel is alsodetailed, with the relevant aspects in measurement inversion. Some preliminary measurements arepresented for Saharan dust intrusion events.Peer ReviewedPostprint (published version

A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes

Introduction: During evolution, blood-feeding arthropods developed a complex salivary mixture that can interfere with host haemostatic and immune response, favoring blood acquisition and pathogen transmission. Therefore, a survey of the salivary gland contents can lead to the identification of molecules with potent pharmacological activity in addition to increase our understanding of the molecular mechanisms underlying the hematophagic behaviour of arthropods. The southern house mosquito, Culex quinquefasciatus, is a vector of several pathogenic agents, including viruses and filarial parasites that can affect humans and wild animals. Results: Previously, a Sanger-based transcriptome of the salivary glands (sialome) of adult C. quinquefasciatus females was published based on the sequencing of 503 clones organized into 281 clusters. Here, we revisited the southern mosquito sialome using an Illumina-based RNA-sequencing approach of both male and female salivary glands. Our analysis resulted in the identification of 7,539 coding DNA sequences (CDS) that were functionally annotated into 25 classes, in addition to 159 long non-coding RNA (LncRNA). Additionally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and/or pathogen transmission. Conclusion: Together, these findings represent an extended reference for the identification and characterization of the proteins containing relevant pharmacological activity in the salivary glands of C. quinquefasciatus mosquitoes.This work was supported by the Intramural Research Program of the Division of Intramural Research (AI001246 and AI000810), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). This work utilized the computational resources of the NIH HPC Biowulf cluster (http://hpc.nih.gov). Open Access funding provided by the National Institutes of Health (NIH).S

Detection of thyroid hormones in human embryonic cavities during the first trimester of pregnancy

et al.Transfer of maternal thyroxine (T4) to the human fetus near term has recently been demonstrated. We investigated whether maternal thyroid hormone is available to the conceptus during the first trimester of pregnancy as well. Transvaginal ultrasound-guided puncture of the embryonic cavities was performed during the first trimester of pregnancy to obtain coelomic fluid between 6 and 11 weeks, and amniotic fluid between 8 and 11 weeks of pregnancy. T4 was found in coelomic fluid with mean values (+/- SEM) being 961 +/- 193 pmol T4/L (747 +/- 150 pg/mL). Concentrations increased both with gestational age and with rising maternal serum T4. Concentrations of 3,5,3'-triiodothyronine (T3) were at least 30 times lower, and those of 3,3',5'-triiodothyronine (rT3) four times higher, than coelomic fluid T4. Thyroxine and rT3 in amniotic fluid (8-11 weeks) were markedly lower than in the coelomic fluid, and T3 was undetectable. These results show that maternal thyroxine can cross the placental barrier as early as the second month of pregnancy. T4 from the coelomic fluid may reach the embryo via the yolk sac. This finding raises the possibility that the increase in maternal T4 occurring during the first trimester may be functionally important for the developing embryo, when its thyroid is not yet functioning.This work was supported by grant 92 / 0888 of Fondo de Investigaciones Sanitarias (Spain) and grant nº 3.4530.93. from the “Fends de la Recherche Scientifique Médicale> (FRSM, Belgium).Peer Reviewe

The salivary gland transcriptome of the neotropical malaria vector Anopheles darlingi reveals accelerated evolution of genes relevant to hematophagy

BackgroundMosquito saliva, consisting of a mixture of dozens of proteins affecting vertebrate hemostasis and having sugar digestive and antimicrobial properties, helps both blood and sugar meal feeding. Culicine and anopheline mosquitoes diverged ~150 MYA, and within the anophelines, the New World species diverged from those of the Old World ~95 MYA. While the sialotranscriptome (from the Greek sialo, saliva) of several species of the Cellia subgenus of Anopheles has been described thoroughly, no detailed analysis of any New World anopheline has been done to date. Here we present and analyze data from a comprehensive salivary gland (SG) transcriptome of the neotropical malaria vector Anopheles darlingi (subgenus Nyssorhynchus).ResultsA total of 2,371 clones randomly selected from an adult female An. darlingi SG cDNA library were sequenced and used to assemble a database that yielded 966 clusters of related sequences, 739 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 183 protein sequences, 114 of which code for putative secreted proteins.ConclusionComparative analysis of sialotranscriptomes of An. darlingi and An. gambiae reveals significant divergence of salivary proteins. On average, salivary proteins are only 53% identical, while housekeeping proteins are 86% identical between the two species. Furthermore, An. darlingi proteins were found that match culicine but not anopheline proteins, indicating loss or rapid evolution of these proteins in the old world Cellia subgenus. On the other hand, several well represented salivary protein families in old world anophelines are not expressed in An. darlingi

An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*

BACKGROUND: Saliva of blood-sucking arthropods contains a cocktail of antihemostatic agents and immunomodulators that help blood feeding. Mosquitoes additionally feed on sugar meals and have specialized regions of their glands containing glycosidases and antimicrobials that might help control bacterial growth in the ingested meals. To expand our knowledge on the salivary cocktail of Ædes ægypti, a vector of dengue and yellow fevers, we analyzed a set of 4,232 expressed sequence tags from cDNA libraries of adult female mosquitoes. RESULTS: A nonredundant catalogue of 614 transcripts (573 of which are novel) is described, including 136 coding for proteins of a putative secretory nature. Additionally, a two-dimensional gel electrophoresis of salivary gland (SG) homogenates followed by tryptic digestion of selected protein bands and MS/MS analysis revealed the expression of 24 proteins. Analysis of tissue-specific transcription of a subset of these genes revealed at least 31 genes whose expression is specific or enriched in female SG, whereas 24 additional genes were expressed in female SG and in males but not in other female tissues. Most of the 55 proteins coded by these SG transcripts have no known function and represent high-priority candidates for expression and functional analysis as antihemostatic or antimicrobial agents. An unexpected finding is the occurrence of four protein families specific to SG that were probably a product of horizontal transfer from prokaryotic organisms to mosquitoes. CONCLUSION: Overall, this paper contributes to the novel identification of 573 new transcripts, or near 3% of the Æ. ægypti proteome assuming a 20,000-protein set, and to the best-described sialome of any blood-feeding insect

Evolutionary fuzzy system for architecture control in a constructive neural network

This work describes an evolutionary system to control the growth of a constructive neural network for autonomous navigation. A classifier system generates Takagi-Sugeno fuzzy rules and controls the architecture of a constructive neural network. The performance of the mobile robot guides the evolutionary learning mechanism. Experiments show the efficiency of the classifier fuzzy system for analyzing if it is worth inserting a new neuron into the architecture

Importance of the reach of the social networks of CIGEFI-UCR: A study of the data according to the publications made between 1979-2015

Desde 1979, el Centro de Investigaciones Geofísicas (CIGEFI) de la Universidad de Costa Rica (UCR) ha evolucionado constantemente hasta convertirse en un referente regional de la Geofísica. Esto ha sido facilitado por diversos actores, investigadores, y colaboradores, tanto propios como de otras afiliaciones. El objetivo de este trabajo fue utilizar las publicaciones elaboradas por el CIGEFI para describir y visualizar las redes sociales de este proceso para el período 1979-2015, mostrando los actores involucrados, las ego-redes más influyentes, la evolución de las redes en tres períodos (1979-1990, 1991-2002 y 2003-2015) para mayor facilidad de procesamiento de la información y las afiliaciones. Se calcularon las medidas de centralidad de grado, cercanía, lejanía y mediación, y se realizaron cinco depuraciones de cada red; para ello, se suprimieron de una a cinco relaciones y actores en cada una. Se concluyó que el trabajo en red fue fundamental para el CIGEFI, pues permitió la integración de un equipo y un abordaje entre distintas disciplinas, clave en los procesos de investigación y toma de decisiones.Since 1979, the Center for Geophysical Research (CIGEFI) of the University of Costa Rica (UCR) has constantly evolved to become a regional reference in geophysics. This has been facilitated by different actors, researchers, and collaborators, both their own and those of other affiliations. The objective of this work was to use the publications elaborated by CIGEFI to describe and visualize the social networks of this process between 1979-2015, showing the actors involved, the most influential ego-networks, the evolution of the networks in three periods (1979-1990, 1991-2002 and 2003-2015), for easier processing of information, and affiliations. The centrality measures of degree, proximity, distance and mediation were calculated, and five network debuggings of each network were carried out; in each one, one to five relationships and actors in each were eliminated. It was concluded that networking was fundamental for CIGEFI, since it allowed the integration of a team and an approach between different disciplines, key in the research and decision making processes.Universidad de Costa Rica/[805-A4-906]/UCR/Costa RicaUniversidad de Costa Rica/[805-B7-286]/UCR/Costa RicaUniversidad de Costa Rica/[805-B6-143]/UCR/Costa RicaUniversidad de Costa Rica/[805-B7-507]/UCR/Costa RicaUniversidad de Costa Rica/[805-A1-715]/UCR/Costa RicaUniversidad de Costa Rica/[805-B0-810]/UCR/Costa RicaConsejo Nacional para Investigaciones Científicas y Tecnológicas/[]/CONICIT/Costa RicaMinisterio de Ciencia, Tecnología y Telecomunicaciones/[]/MICITT/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones Geofísicas (CIGEFI)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencias del Mar y Limnología (CIMAR)UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Físic